Extracellular Proteomes of Arabidopsis Thaliana and Brassica Napus Roots: Analysis and Comparison by MudPIT and LC-MS/MS

by Urmila Basu. Jennafer L. Francis. Randy M. Whittal. Julie L. Stephens. Yang Wang. Osmar R. Zaiane. Randy Goebel. Douglas G. Muench. Allen G. Good and Gregory J. Taylor.

Plant and Soil, Volume 286, Numbers 1-2

An important principle of the functional organization of plant cells is the targeting of proteins to specific subcellular locations. The physical location of proteins within the apoplasm/rhizosphere at the root–soil interface positions them to play a strategic role in plant response to biotic and abiotic stress. We previously demonstrated that roots of Triticum aestivum and Brassica napus exude a large suite of proteins to the apoplasm/rhizosphere [Basu et al. (1994) Plant Physiol 106:151–158; Basu et al. (1999) Physiol Plant 106:53–61]. This study is a first step to identify low abundance extracytosolic proteins from Arabidopsis thaliana and Brassica napus roots using recent advances in the field of proteomics. [via]

http://www.cs.ualberta.ca/~zaiane/postscript/...

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Extracellular Proteomes of Arabidopsis Thaliana and Brassica Napus Roots: Analysis and Comparison by MudPIT and LC-MS/MS (2006)

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