Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion Using the ProteoSpin Detergent Clean-Up Kit

This application note evaluates the ProteoSpin™ Detergent Clean-up Micro Kit (P/N 10500) for the removal of a biological detergent present in a protein solution, and demonstrates the kit’s efficiency in preparing a purified protein for downstream applications such as mass spectrometry. This evaluation also establishes that detergent removal is a necessary step for subsequent proteolytic digestion of the protein with trypsin, and that these results are more easily achieved with the ProteoSpin™ kit than by ultrafiltration with a molecular cut- off membrane. OVERVIEW The removal of detergents from protein samples is often a necessary step before many downstream applications, particularly those that involve structural analysis including mass spectrometry. A number of conventional methods for detergent removal are available including dialysis, gel filtration chromatography, hydrophobic interaction chromatography and ultrafiltration. Recently, a limited number of kits for detergent removal have been introduced to the market. [via]

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Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion Using the ProteoSpin Detergent Clean-Up Kit

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